Journal: Scientific Reports

Article Title: Male mice, caged in the International Space Station for 35 days, sire healthy offspring

doi: 10.1038/s41598-019-50128-w

Figure Lengend Snippet: Characterization of fertilized eggs and F1 mice from AG and MG males. ( a ) Embryogenesis of fertilized eggs. Embryogenesis was observed for 4 days after IVF. ( b ) Immunostaining of OCT4 and CDX2 in blastocysts. OCT4 and CDX2 were used as markers for ICM and TE cells in blastocysts, respectively. Scale bars are 100 µm. (c) Quantification of OCT4- and CDX2-positive cells in blastocysts. Data are the mean ± standard deviation. N.S.: not significant. ( d ) Rates of delivered pups. Fertilized eggs obtained by IVF were transferred into pseudopregnent females. ( e ) Offspring from fertilized eggs with MG spermatozoa. ( f ) Growth curve of F1 mice. Body weight was monitored by postnatal day 63. “M” and “F” show the male and female mice, respectively. ( g ) Litter sizes of F1 × F1 intercrosses. Data are the mean ± standard deviation. N.S.: not significant.

Article Snippet: Specifically, rabbit anti-mouse OCT3/4 polyclonal antibody (a marker for ICM cells, 1:200, MBL Code No. PM048) and mouse anti-CDX2 monoclonal antibody (a maker for TE cells, 1:150, MBL Code No. B-MU392AUC) were used as the primary antibodies.

Techniques: Immunostaining, Standard Deviation

Journal: Advanced Science

Article Title: Multifocal Organoid Capturing of Colon Cancer Reveals Pervasive Intratumoral Heterogenous Drug Responses

doi: 10.1002/advs.202103360

Figure Lengend Snippet: Antibodies used for immunohistochemistry

Article Snippet: Mouse monoclonal anti‐CDX2 , BioGenex , Cat# AM392; RRID:AB_2650531 , 1:300.

Techniques: